Giessener Elektronische Bibliothek

GEB - Giessener Elektronische Bibliothek

Aphid gel saliva : Sheath structure, protein composition and secretory dependence on stylet-tip milieu

Will, Torsten ; Steckbauer, Kathrin ; Hardt, Martin ; van Bel, Aart J. E.


Originalveröffentlichung: (2012) PLoS ONE, 7(10), e46903, 1-8 doi:10.1371/journal.pone.0046903
Zum Volltext im pdf-Format: Dokument 1.pdf (611 KB)


Bitte beziehen Sie sich beim Zitieren dieses Dokumentes immer auf folgende
URN: urn:nbn:de:hebis:26-opus-90731
URL: http://geb.uni-giessen.de/geb/volltexte/2012/9073/

Bookmark bei Connotea Bookmark bei del.icio.us


Freie Schlagwörter (Englisch): aphids , saliva secretion , watery / gel saliva , stylet milieu , oxygen dependence
Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universität Gießen
Institut: Plant Cell Biology Research Group
Fachgebiet 1: Zentrale Biotechnische Betriebseinheit
Fachgebiet 2: Biologie
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2012
Publikationsdatum: 20.11.2012
Kurzfassung auf Englisch: In order to separate and analyze saliva types secreted during stylet propagation and feeding, aphids were fed on artificial diets. Gel saliva was deposited as chains of droplets onto Parafilm membranes covering the diets into which watery saliva was secreted. Saliva compounds collected from the diet fluid were separated by SDS-PAGE, while non-soluble gel saliva deposits were processed in a novel manner prior to protein separation by SDS-PAGE. Soluble (watery saliva) and non-soluble (gel saliva) protein fractions were significantly different. To test the effect of the stylet milieu on saliva secretion, aphids were fed on various diets. Hardening of gel saliva is strongly oxygen-dependent, probably owing to formation of sulfide bridges by oxidation of sulphydryl groups. Surface texture of gel saliva deposits is less pronounced under low-oxygen conditions and disappears in dithiothreitol containing diet. Using diets mimicking sieve-element sap and cell-wall fluid respectively showed that the soluble protein fraction was almost exclusively secreted in sieve elements while non-soluble fraction was preferentially secreted at cell wall conditions. This indicates that aphids are able to adapt salivary secretion in dependence of the stylet milieu.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung