Interaction of two photoreceptors in the regulation of bacterial photosynthesis genes
Metz, Sebastian ;
Haberzettl, Kerstin ;
Frühwirth, Sebastian ;
Teich, Kristin ;
Hasewinkel, Christian ;
Klug, Gabriele
Originalveröffentlichung:
(2012) Nucleic Acids Research, 40(13), 5901-5909 doi:10.1093/nar/gks243
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URN: urn:nbn:de:hebis:26-opus-86916
URL: http://geb.uni-giessen.de/geb/volltexte/2012/8691/
Freie Schlagwörter (Englisch):
expression of photosynthesis genes , gene regulation , Rhodobacter sphaeroides , photoreceptor proteins AppA / CryB , transcriptional repressor PpsR
Sammlung:
Open Access - Publikationsfonds
Universität
Justus-Liebig-Universität Gießen
Institut:
Institut für Mikrobiologie und Molekularbiologie
Fachgebiet:
Biochemie (FB 08)
DDC-Sachgruppe:
Biowissenschaften, Biologie
Dokumentart:
Aufsatz
Sprache:
Englisch
Erstellungsjahr:
2012
Publikationsdatum:
04.04.2012
Kurzfassung auf Englisch:
The expression of photosynthesis genes in the facultatively photosynthetic bacterium Rhodobacter sphaeroides is controlled by the oxygen tension and by light quantity. Two photoreceptor proteins, AppA and CryB, have been identified in the past, which are involved in this regulation. AppA senses light by its N-terminal BLUF domain, its C-terminal part binds heme and is redox-responsive. Through its interaction to the transcriptional repressor PpsR the AppA photoreceptor controls expression of photosynthesis genes. The cryptochrome-like protein CryB was shown to affect regulation of photosynthesis genes, but the underlying signal chain remained unknown. Here we show that CryB interacts with the C-terminal domain of AppA and modulates the binding of AppA to the transcriptional repressor PpsR in a light-dependent manner. Consequently, binding of the transcription factor PpsR to its DNA target is affected by CryB. In agreement with this, all genes of the PpsR regulon showed altered expression levels in a CryB deletion strain after blue-light illumination. These results elucidate for the first time how a bacterial cryptochrome affects gene expression.
Lizenz:
Creative Commons - Namensnennung, Nicht kommerziell, Keine Bearbeitung
