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URN: urn:nbn:de:hebis:26-opus-50978
URL: http://geb.uni-giessen.de/geb/volltexte/2007/5097/


The Functional Importance of CD177 on Neutrophil in Interaction with Endothelium

Maniar, Amudhan


Originalveröffentlichung: (2007) Giessen : VVB Laufersweiler 2007
pdf-Format: Dokument 1.pdf (2.537 KB)

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Universität Justus-Liebig-Universität Gießen
Institut: Institute for Clinical Immunology and Transfusion Medicine Medical School
Fachgebiet: Biologie
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: Dissertation
Zeitschrift, Serie: Edition scientifique
ISBN / ISSN: 978-3-8359-5211-0
Sprache: Englisch
Tag der mündlichen Prüfung: 10.10.2007
Erstellungsjahr: 2007
Publikationsdatum: 23.11.2007
Kurzfassung auf Englisch: This study sheds light on the characterisation of CD177 and identification of CD177s counter receptor on endothelium. We could demonstrate that PECAM-1 on endothelial cells functions directly as the counter receptor for CD177 by following observations;


1. Our recombinant CD177-Fc fusion protein, which was produced in insect cells, reacted with 130 KDa molecules on endothelium.

2. Screening with different mabs identified the 130 KDa protein as endothelial PECAM-1.

3. Biochemical and immunological studies using recombinant CD177-Fc fusion protein confirmed that endothelial PECAM-1 functions directly as the counter-receptor for CD177.

4. Functional studies in vitro demonstrated that purified CD177 protein inhibits
CD177 mediated cell adhesion to immobilized PECAM-1 protein as well as neutrophil migration through endothelial cells.


These results indicate that CD177 can act as cell adhesion molecule involving neutrophil-endothelial interaction. This observation may provide a new concept in understanding the multi-step process of transendothelial migration.
In the future, this concept should be studied in different in vitro as well as in vivo model in more detail.