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Application of recombinant DNA technology to studies on plant secondary metabolism

Zárate, Rafael ; Yeoman, Michael M.

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Universität Justus-Liebig-Universität Gießen
Institut: Instituto Universitario de Bio-Orgánica A. González, Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain. Institute of Cell and Molecular Biology, Centre for Plant Science, The University of Edinburgh, Edinburgh, Scotland, UK
Fachgebiet: Haushalts- und Ernährungswissenschaften - Ökotrophologie
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2001
Publikationsdatum: 23.09.2003
Kurzfassung auf Englisch: This review which is concerned with the application of recombinant DNA technology to studies on plant secondary metabolism, presents the more common plant transformation strategies and shows how these genetic approaches are being used in attempts to manipulate and increase the yield of secondary metabolites, both in cultures and in transformed plants.
The different plant transformation strategies reviewed here are: infection with intact Agrobacteria; particle bombardment, vacuum infiltration and floral dip; viral vectors and finally protoplast fusion. The review continues with examples of the application of several of these transformation strategies in the manipulation of secondary metabolism. These are outlined under four subheadings which include developmentally regulated genes, addition of novel genes, down-regulation of specific genes and insertion of regulatory genes. Finally, under concluding remarks, reference is made to the advances achieved in the manipulation of
plant secondary metabolism and how these approaches may impact on this new.