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URN: urn:nbn:de:hebis:26-opus-856
URL: http://geb.uni-giessen.de/geb/volltexte/1999/85/


Pharmakologische Modulation des "oxidative burst" aktivierter neutrophiler Granulozyten in Anwesenheit von anoxischen Kardiomyozyten

Szalay, Susanne


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Universität Justus-Liebig-Universität Gießen
Institut: Max-Planck-Institut für Physiologische und Klinische Forschung, Kerckhoff-Institut, Abt. Experimentelle Kardiologie, Bad Nauheim
Fachgebiet: Medizin
DDC-Sachgruppe: Medizin
Dokumentart: Dissertation
Sprache: Deutsch
Tag der mündlichen Prüfung: 04.05.1999
Erstellungsjahr: 1997
Publikationsdatum: 17.11.1999
Kurzfassung auf Englisch: Reoxygenation of ischemic myocardium may be harmful due to the formation of free radicals. Therefore, experiments were carried out with a co-incubation of
isolated myocytes and isolated neutrophils from rats. Myocytes were kept for 12h in anoxic conditions. Neutrophils were stimulated with N-formyl
Met-Leu-Phe (fMLP). The free radical burst (FRB) was detected by a luminol mediated bioluminescence assay. Anoxic myocytes rapidly increase the FRB of
activated neutrophils to about 844% compared to normoxic myocytes as controls. The FRB can be reduced by addition of superoxid-dismutase (SOD),
catalase and gluthation-peroxidase to about 85%, 77% and 72% of the 12h anoxic myocyte group. A further reduction was observed when SOD and catalase
together were added (93%). Similary, treatment of normoxic myocytes by diethyldithiocarbamate (a SOD inhibitor) increases the burst formation to about
200% of the control group. The formation of the FRB in the presence of 12h anoxic myocytes was also decreased by addition of isoproterenol (54%) and
verapamil (45%). Adenosine agonists like N6-2-phenylisopropyladenosine, 5`N-ethylcarboxamidoadenosine and cyclopentyl-adenosine can also reduce the
fMLP activated neutrophil FRB to about 86%, 77% and 70%, respectively. In conclusion, anoxic myocytes have a better chance of survival in co-culture with
activated neutrophils when substances which reduce the free radical formation by different pathways are added.