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Urinary excretion of in vivo 13C-labelled milk oligosaccharides in breastfed infants

Rudloff, Silvia ; Pohlentz, Gottfried ; Borsch, Christian ; Lentze, Michael J. ; Kunz, Clemens

Originalveröffentlichung: (2012) British Journal of Nutrition 107(07):957-963 doi:10.1017/S0007114511004016
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URN: urn:nbn:de:hebis:26-opus-98980

Sammlung: Allianz-/Nationallizenzen / Artikel
Universität Justus-Liebig-Universit√§t Gie√üen
Institut: Department of Pediatrics; Institute of Nutritional Science
Fachgebiet: Haushalts- und Ern√§hrungswissenschaften - √Ėkotrophologie
DDC-Sachgruppe: Medizin
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2012
Publikationsdatum: 10.07.2013
Kurzfassung auf Englisch: Recent observations indicate that human milk oligosaccharides (HMO) are involved in a variety of physiological processes in infants. Their metabolic fate, however, is virtually unknown. We investigated metabolic aspects in infants after endogenous 13C-labelling of HMO. An oral bolus of natural and 13C-labelled galactose (Gal; 23¬†g Gal+4¬†g 13C-Gal) was given to ten lactating women. Aliquots of milk at each nursing as well as breath samples from the mothers and urine from their infants were collected over 36¬†h. The 13C-enrichment of HMO and their renal excretion was determined by isotope ratio-MS; characterisation was achieved by fast atom bombardment-MS. After the Gal bolus was given, an immediate 13C-enrichment in milk and in infants¬ī urine was observed which lasted 36¬†h. Mass spectrometric analysis of 13C-enriched urinary fractions confirmed the excretion of a variety of neutral and acidic HMO without metabolic modification of their structures. Components with glucose split off at the reducing end were also detectable. Quantitative data regarding the infants¬ī intake of lacto-N-tetraose and its monofucosylated derivative lacto-N-fucopentaose II ranged from 50 to 160¬†mg with each suckling, respectively; renal excretion of both components varied between 1 and 3¬†mg/d. Since the intake of individual HMO by the infants was in the range of several hundred mg per suckling, i.e. several g/d, and some of these components were excreted in mg amounts as intact HMO with the infants¬ī urine, not only local but also systemic effects might be expected.
Lizenz: Allianz- / Nationallizenz