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Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins

Arneth, Borros M.


Originalveröffentlichung: (2018) PeerJ 6:e4462 doi: 10.7717/peerj.4462
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URN: urn:nbn:de:hebis:26-opus-153971
URL: http://geb.uni-giessen.de/geb/volltexte/2020/15397/


Freie Schlagwörter (Englisch): regulatory T cells , CD4+ T helper cells , lymphocytes , CD8+ cytotoxic T cells , interferon-gamma
Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universit√§t Gie√üen
Institut: Institute of Laboratory Medicine and Pathobiochemistry, Molecular Diagnostics
Fachgebiet: Medizin
DDC-Sachgruppe: Medizin
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2018
Publikationsdatum: 18.08.2020
Kurzfassung auf Englisch: This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the expression of extracellular activation markers on and intracellular cytokines in T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with beta-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extra-cellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung 4.0