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High-resolution atmospheric-pressure MALDI mass spectrometry imaging workflow for lipidomic analysis of late fetal mouse lungs

Garikapati, Vannuruswamy ; Karnati, Srikanth ; Bhandari, Dhaka Ram ; Baumgart-Vogt, Eveline ; Spengler, Bernhard

Originalveröffentlichung: (2019) Scientific Reports 9:3192 doi: 10.1038/s41598-019-39452-3
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URN: urn:nbn:de:hebis:26-opus-149001

Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universit√§t Gie√üen
Institut: Institute of Inorganic and Analytical Chemistry
Fachgebiet: Chemie
DDC-Sachgruppe: Chemie
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2019
Publikationsdatum: 22.10.2019
Kurzfassung auf Englisch: Mass spectrometry imaging (MSI) provides label-free, non-targeted molecular and spatial information of the biomolecules within tissue. Lipids play important roles in lung biology, e.g. as surfactant, preventing alveolar collapse during normal and forced respiration. Lipidomic characterization of late fetal mouse lungs at day 19 of gestation (E19) has not been performed yet. In this study we employed high-resolution atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization MSI for the lipidomic analysis of E19 mouse lungs. Molecular species of different lipid classes were imaged in E19 lung sections at high spatial and mass resolution in positive- and negative-ion mode. Lipid species were characterized based on accurate mass and on-tissue tandem mass spectrometry. In addition, a dedicated sample preparation protocol, homogenous deposition of matrices on tissue surfaces and data processing parameters were optimized for the comparison of signal intensities of lipids between different tissue sections of E19 lungs of wild type and Pex11beta-knockout mice. Our study provides lipid information of E19 mouse lungs, optimized experimental and data processing strategies for the direct comparison of signal intensities of metabolites (lipids) among the tissue sections from MSI experiments. To best of our knowledge, this is the first MSI and lipidomic study of E19 mouse lungs.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung 4.0