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C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation

Richter, Katrin ; Sagawe, Sabrina ; Hecker, Andreas ; Küllmar, Mira ; Askevold, Ingolf ; Damm, Jelena ; Heldmann, Sarah ; Pöhlmann, Michael ; Ruhrmann, Sophie ; Sander, Michael ; Schlüter, Klaus-Dieter ; Wilker, Sigrid ; König, Inke R. ; Kummer, Wolfgang ; Padberg, Winfried ; Hone, Arik J. ; McIntosh, J. Michael ; Zakrzewicz, Anna Teresa ; Koch, Christian ; Grau, Veronika

Originalveröffentlichung: (2018) Frontiers in Immunology 9(1604) doi: 10.3389/fimmu.2018.01604
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URN: urn:nbn:de:hebis:26-opus-146319

Freie Schlagwörter (Englisch): C-reactive protein , interleukin-1β , NLRP3 inflammasome , monocytes , nicotinic acetylcholine receptors
Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universität Gießen
Institut: Department of General and Thoracic Surgery
Fachgebiet: Medizin
DDC-Sachgruppe: Medizin
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2018
Publikationsdatum: 20.05.2019
Kurzfassung auf Englisch: Blood levels of the acute phase reactant C-reactive protein (CRP) are frequently measured as a clinical marker for inflammation, but the biological functions of CRP are still controversial. CRP is a phosphocholine (PC)-binding pentraxin, mainly produced in the liver in response to elevated levels of interleukin-1beta (IL-1beta) and of the IL-1beta-dependent cytokine IL-6. While both cytokines play important roles in host defense, excessive systemic IL-1beta levels can cause life-threatening diseases such as trauma-associated systemic inflammation. We hypothesized that CRP acts as a negative feed back regulator of monocytic IL-1beta maturation and secretion. Here, we demonstrate that CRP, in association with phosphocholine, efficiently reduces ATP-mediated inflammasome activation and IL-1β release from human peripheral blood mononuclear leukocytes and monocytic U937 cells. Effective concentrations are in the range of marginally pathologic CRP levels (IC50 = 4.9 µg/ml). CRP elicits metabotropic functions at nicotinic acetylcholine receptors (nAChR) containing subunits alpha7, alpha9 and alpha10 and suppresses the function of ATP-sensitive P2X7 receptors in monocytic cells. Of note, CRP does not induce ion currents at conventional nAChR, suggesting that CRP is a potent nicotinic agonist controlling innate immunity without entailing the risk of adverse effects in the nervous system. In a prospective study on multiple trauma patients, IL-1beta plasma concentrations negatively correlated with preceding CRP levels, whereas inflammasome-independent cytokines IL 6 and TNF-alpha positively correlated. In conclusion, phosphocholine-laden CRP is an unconventional nicotinic agonist that potently inhibits ATP-induced inflammasome activation and has the potential to protect against trauma-associated sterile inflammation.
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