Giessener Elektronische Bibliothek

GEB - Giessener Elektronische Bibliothek

Bacterial ribonuclease binase exerts an intra-cellular anti-viral mode of action targeting viral RNAs in influenza a virus-infected MDCK-II cells

Shah Mahmud, Raihan ; Mostafa, Ahmed ; Müller, Christin ; Kanrai, Pumaree ; Ulyanova, Vera ; Sokurenko, Yulia ; Dzieciolowski, Julia ; Kuznetsova, Irina ; Ilinskaya, Olga ; Pleschka, Stephan

Originalveröffentlichung: (2018) Virology Journal 15:5 doi: 10.1186/s12985-017-0915-1
Zum Volltext im pdf-Format: Dokument 1.pdf (915 KB)

Bitte beziehen Sie sich beim Zitieren dieses Dokumentes immer auf folgende
URN: urn:nbn:de:hebis:26-opus-146022

Freie Schlagwörter (Englisch): Ribonuclease , RNase , Binase , Influenza virus , Anti-viral activity
Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universität Gießen
Institut: Institute of Medical Virology
Fachgebiet: Medizin fachübergreifend
DDC-Sachgruppe: Medizin
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2018
Publikationsdatum: 20.05.2019
Kurzfassung auf Englisch: Background: Influenza is a severe contagious disease especially in children, elderly and immunocompromised patients. Beside vaccination, the discovery of new anti-viral agents represents an important strategy to encounter seasonal and pandemic influenza A virus (IAV) strains. The bacterial extra-cellular ribonuclease binase is a well-studied RNase from Bacillus pumilus. Treatment with binase was shown to improve survival of laboratory animals infected with different RNA viruses. Although binase reduced IAV titer in vitro and in vivo, the mode of action (MOA) of binase against IAV at the molecular level has yet not been studied in depth and remains elusive. Methods To analyze whether binase impairs virus replication by direct interaction with the viral particle we applied a hemagglutination inhibition assay and monitored the integrity of the viral RNA within the virus particle by RT-PCR. Furthermore, we used Western blot and confocal microscopy analysis to study whether binase can internalize into MDCK-II cells. By primer extension we examined the effect of binase on the integrity of viral RNAs within the cells and using a mini-genome system we explored the effect of binase on the viral expression.
Results: We show that (i) binase does not to attack IAV particle-protected viral RNA, (ii) internalized binase could be detected within the cytosol of MDCK-II cells and that (iii) binase impairs IAV replication by specifically degrading viral RNA species within the infected MDCK-II cells without obvious effect on cellular mRNAs.
Conclusion: Our data provide novel evidence suggesting that binase is a potential anti-viral agent with specific intra-cellular MOA.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung 4.0