Giessener Elektronische Bibliothek

GEB - Giessener Elektronische Bibliothek

Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging

Kolecka, Malgorzata Anna ; Arnhold, Stefan ; Schmidt, Martin ; Reich, Christine ; Kramer, Martin ; Failing, Klaus ; von PĂŒckler, Kerstin

Originalveröffentlichung: (2017) BMC Veterinary Research 13:62 doi: 10.1186/s12917-017-0980-0
Zum Volltext im pdf-Format: Dokument 1.pdf (1.998 KB)

Bitte beziehen Sie sich beim Zitieren dieses Dokumentes immer auf folgende
URN: urn:nbn:de:hebis:26-opus-128762

Freie Schlagwörter (Englisch): Canine adipose-derived mesenchymal stem cells , Superparamagnetic iron oxide particles , Endorem , Magnetic resonance
Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-UniversitĂ€t Gießen
Institut: Department of Veterinary Clinical Sciences
Fachgebiet: VeterinÀrmedizin
DDC-Sachgruppe: Landwirtschaft
Dokumentart: Aufsatz
Sprache: Deutsch
Erstellungsjahr: 2017
Publikationsdatum: 26.05.2017
Kurzfassung auf Englisch: Background: Therapy with mesenchymal stem cells (MSCs) has been reported to provide beneficial effects in the treatment of neurological and orthopaedic disorders in dogs. The exact mechanism of action is poorly understood. Magnetic resonance imaging (MRI) gives the opportunity to observe MSCs after clinical administration. To visualise MSCs with the help of MRI, labelling with an MRI contrast agent is necessary. However, it must be clarified whether there is any negative influence on cell function and viability after labelling prior to clinical administration. Results: For the purpose of the study, seven samples with canine adipose-derived stem cells were incubated with superparamagnetic iron oxide nanoparticles (SPIO: 319.2 ”g/mL Fe) for 24 h. The internalisation of the iron particles occurred via endocytosis. SPIO particles were localized as free clusters in the cytoplasm or within lysosomes depending on the time of investigation. The efficiency of the labelling was investigated using Prussian blue staining and MACS assay. After 3 weeks the percentage of SPIO labelled canine stem cells decreased. Phalloidin staining showed no negative effect on the cytoskeleton. Labelled cells underwent osteogenic and adipogenic differentiation. Chondrogenic differentiation occurred to a lesser extent compared with a control sample. MTT-Test and wound healing assay showed no influence of labelling on the proliferation. The duration of SPIO labelling was assessed using a 1 Tesla clinical MRI scanner and T2 weighted turbo spin echo and T2 weighted gradient echo MRI sequences 1, 2 and 3 weeks after labelling. The hypointensity caused by SPIO lasted for 3 weeks in both sequences. Conclusions: An Endorem labelling concentration of 319.2 ”g/mL Fe (448 ”g/mL SPIO) had no adverse effects on the viability of canine ASCs. Therefore, this contrast agent could be used as a model for iron oxide labelling agents. However, the tracking ability in vivo has to be evaluated in further studies.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung 4.0