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Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV

Oellers, Dana E. ; Bauer, Natali ; Ginder, Melanie ; Johannes, Sigrid ; Pernecker, Iris ; Moritz, Andreas


Originalveröffentlichung: (2016) BMC Veterinary Research 12:148 doi: 10.1186/s12917-016-0779-4
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URN: urn:nbn:de:hebis:26-opus-128733
URL: http://geb.uni-giessen.de/geb/volltexte/2017/12873/


Freie Schlagwörter (Englisch): RNA-rich platelets , Canine , Hematology analyzer
Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universität GieĂźen
Institut: Department of Veterinary Clinical Sciences
Fachgebiet: Veterinärmedizin
DDC-Sachgruppe: Landwirtschaft
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2016
Publikationsdatum: 26.05.2017
Kurzfassung auf Englisch: Background: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30–38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate (“Oellers-gate”) and to establish reference intervals (RIs) in>120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. Results: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the “Oellers-gate”. Good correlation was found between the different gates (rs: 0.88–1.00). RIs for the “Pankraz-gate”, the “Gelain-gate”, and the “Oellers-gate” were 0.0–1.2, 0.2–3.7 and 0.2–3.9 % respectively. CVs were ranging between 22 and 41 %. Conclusions: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements.
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