Inhibition of Low-Grade Inflammation by Anthocyanins after Microbial Fermentation in Vitro
Kuntz, Sabine ;
Kunz, Clemens ;
Domann, Eugen ;
Würdemann, Nora ;
Unger, Franziska ;
Römpp, Andreas ;
Rudloff, Silvia
Originalveröffentlichung:
(2016) Nutrients 8(7):411 doi: 10.3390/nu8070411
Zum Volltext im pdf-Format:
Dokument 1.pdf (2.032 KB)
Bitte beziehen Sie sich beim Zitieren dieses Dokumentes immer auf folgende
URN: urn:nbn:de:hebis:26-opus-124702
URL: http://geb.uni-giessen.de/geb/volltexte/2017/12470/
Freie Schlagwörter (Englisch):
grapes and berries , anthocyanins , low-grade inflammation , fermentation , in vitro co-culture model
Sammlung:
Open Access - Publikationsfonds
Universität
Justus-Liebig-Universität Gießen
Institut:
Institute of Nutritional Science
Fachgebiet:
Haushalts- und Ernährungswissenschaften - Ökotrophologie
DDC-Sachgruppe:
Biowissenschaften, Biologie
Dokumentart:
Aufsatz
Sprache:
Englisch
Erstellungsjahr:
2016
Publikationsdatum:
03.02.2017
Kurzfassung auf Englisch:
The anti-inflammatory effects of anthocyanins (ACNs) on vascular functions are discussed controversially because of their low bioavailability. This study was performed to determine whether microorganism (MO)-fermented ACNs influence vascular inflammation in vitro. Therefore, MO growth media were supplemented with an ACN-rich grape/berry extract and growth responses of Escherichia coli, E. faecalis and H. alvei, as well as ACN fermentation were observed. MO supernatants were used for measuring the anti-inflammatory effect of MO-fermented ACNs in an epithelial-endothelial co-culture transwell system. After basolateral enrichment (240 min), endothelial cells were stimulated immediately or after 20 h with TNF-a. Afterwards, leukocyte adhesion, expression of adhesion molecules and cytokine release were measured. Results indicate that E. coli, E. faecalis and H. alvei utilized ACNs differentially concomitant with different anti-inflammatory effects. Whereas E. coli utilized ACNs completely, no anti-inflammatory effects of fermented ACNs were observed on activated endothelial cells. In contrast, ACN metabolites generated by E. faecalis and H. alvei significantly attenuated low-grade stimulated leukocyte adhesion, the expression of adhesion molecules E-selectin, VCAM-1 and ICAM-1 and cytokine secretion (IL-8 and IL-6), as well as NF-KB mRNA expression with a more pronounced effect of E. faecalis than H. alvei. Thus, MO-fermented ACNs have the potential to reduce inflammation.
Lizenz:
Creative Commons - Namensnennung 4.0