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CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

Schneider, Tim ; Hung, Lee-Hsueh ; Schreiner, Silke ; Starke, Stefan ; Eckhof, Heinrich ; Rossbach, Oliver ; Reich, Stefan ; Medenbach, Jan ; Bindereif, Albrecht


Originalveröffentlichung: (2016) Scientific Reports 6:31313 doi: 10.1038/srep31313
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URN: urn:nbn:de:hebis:26-opus-124646
URL: http://geb.uni-giessen.de/geb/volltexte/2017/12464/

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Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universität Gießen
Institut: Institute of Biochemistry
Fachgebiet: Biochemie (FB 08)
DDC-Sachgruppe: Chemie
Dokumentart: Aufsatz
Sprache: Englisch
Erstellungsjahr: 2016
Publikationsdatum: 03.02.2017
Kurzfassung auf Englisch: Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-co-immunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung 4.0