Giessener Elektronische Bibliothek

GEB - Giessener Elektronische Bibliothek

Phosphocholine – an agonist of metabotropic but not of ionotropic functions of alpha9-containing nicotinic acetylcholine receptors

Richter, Katrin ; Mathes, V. ; Fronius, M. ; Althaus, M. ; Hecker, Andreas ; Krasteva-Christ, G. ; Padberg, Winfried ; Hone, A. J. ; McIntosh, J. M. ; Zakrzewicz, Anna ; Grau, Veronika


Originalveröffentlichung: (2016) Scientific Reports 6:28660 doi: 10.1038/srep28660
Zum Volltext im pdf-Format: Dokument 1.pdf (1.154 KB)


Bitte beziehen Sie sich beim Zitieren dieses Dokumentes immer auf folgende
URN: urn:nbn:de:hebis:26-opus-124628
URL: http://geb.uni-giessen.de/geb/volltexte/2017/12462/


Sammlung: Open Access - Publikationsfonds
Universität Justus-Liebig-Universität GieĂźen
Institut: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery
Fachgebiet: Medizin
DDC-Sachgruppe: Medizin
Dokumentart: Aufsatz
Sprache: Deutsch
Erstellungsjahr: 2016
Publikationsdatum: 03.02.2017
Kurzfassung auf Englisch: We demonstrated previously that phosphocholine and phosphocholine-modified macromolecules efficiently inhibit ATP-dependent release of interleukin-1beta from human and murine monocytes by a mechanism involving nicotinic acetylcholine receptors (nAChR). Interleukin-1beta is a potent pro-inflammatory cytokine of innate immunity that plays pivotal roles in host defence. Control of interleukin-1beta release is vital as excessively high systemic levels cause life threatening inflammatory diseases. In spite of its structural similarity to acetylcholine, there are no other reports on interactions of phosphocholine with nAChR. In this study, we demonstrate that phosphocholine inhibits ion-channel function of ATP receptor P2X7 in monocytic cells via nAChR containing alpha9 and alpha10 subunits. In stark contrast to choline, phosphocholine does not evoke ion current responses in Xenopus laevis oocytes, which heterologously express functional homomeric nAChR composed of alpha9 subunits or heteromeric receptors containing alpha9 and alpha10 subunits. Preincubation of these oocytes with phosphocholine, however, attenuated choline-induced ion current changes, suggesting that phosphocholine may act as a silent agonist. We conclude that phophocholine activates immuno-modulatory nAChR expressed by monocytes but does not stimulate canonical ionotropic receptor functions.
Lizenz: Lizenz-Logo  Creative Commons - Namensnennung 4.0