TY - THES T1 - Phenotypic and genotypic characteristics of bacteria of genus Arcanobacterium with the emphasis on Arcanobacterium phocae isolated from epidemic necrotic pyoderma of fur animals T3 - Giessen : VVB Laufersweiler Verlag A1 - Alssahen,Mazen Y1 - 2021/10/19 N2 - In the present study A. phocae strains isolated from fur animals in Finland, including foxes, minks and finnraccoons and nine additional A. phocae strains isolated from cases of mink dermatitis of a single farm in Finland were identified phenotypically and genotypically, the nine strains from a single farm also for epidemiological relationships by whole genome sequencing and single nucleotide polymorphism analysis. The strains were characterized by determination of cultural properties, their hemolysis, by detection of synergistic and antagonistic CAMP-like hemolytic reactions, by determination of various biochemical properties and by investigating their peptidic profiles by MALDI-TOF MS and FT-IR analyses. A molecular identification of the A. phocae strains was performed by sequencing the 16S rRNA gene and by amplification and sequencing of phocaelysin encoding gene phl as novel target. Gene phl could also be used as target gene in a newly developed LAMP-assay. However, the putative virulence factor phocaelysin (PHL) of A. phocae seems to be present in all A. phocae strains and might play a role for pathogenicity of this bacterial species. The nine A. phocae strains from a single farm were additionally characterized genotypically by sequencing the elongation factor tu encoding gene tuf and RNA polymerase encoding gene rpoB and by whole genome sequencing and single nucleotide polymorphism analysis. The latter and part of the other investigations revealed that the nine A. phocae strains from a single farm might have developed from a common ancestor. All the data of the present study will help to identify A. phocae in future and might help to understand the pathogenic importance of this bacterial species in fur animals and in other animal species and possibly in humans. CY - Gießen PB - Universitätsbibliothek SN - 978-3-8359-2184-9 AD - Otto-Behaghel-Str. 8, 35394 Gießen UR - http://geb.uni-giessen.de/geb/volltexte/2021/16108 ER -